LeHavreV1, Main, Exploration, bibRecord, 000463

P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods

Identifieur interne : 000463 ( Main/Exploration ); précédent : 000462; suivant : 000464

P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods

Auteurs : Jennifer Pasquier [France, États-Unis, Qatar] ; Damien Rioult [France] ; Nadine Abu-Kaoud [Qatar] ; Sabine Marie [France] ; Arash Rafii [États-Unis, Qatar] ; Bella S. Guerrouahen [États-Unis, Qatar] ; Frank Le Foll [France]

Source :

BioMed Research International [ 2314-6133 ] ; 2013.

RBID : PMC:3848087

English descriptors

KwdEn :
- Cell Line, Tumor, Drug Resistance, Multiple (physiology), Drug Resistance, Neoplasm (physiology), Fluoresceins (metabolism), Fluorescence, Humans, MCF-7 Cells, P-Glycoproteins (metabolism), Reproducibility of Results.
MESH :
- chemical , metabolism : Fluoresceins, P-Glycoproteins.
- physiology : Drug Resistance, Multiple, Drug Resistance, Neoplasm.
- Cell Line, Tumor, Fluorescence, Humans, MCF-7 Cells, Reproducibility of Results.

Abstract

Background. P-gp expression has been linked to the efflux of chemotherapeutic drugs in human cancers leading to multidrug resistance. Fluorescence techniques have been widely applied to measure the P-gp activity. In this paper, there is a comparison between the advantages of two fluorescence approaches of commonly available and affordable instruments: the microplate reader (MPR) and the flow cytometer to detect the P-gp efflux activity using calcein-AM. Results. The selectivity, sensibility, and reproducibility of the two methods have been defined. Our results showed that the MPR is more powerful for the detection of small inhibition, whereas the flow cytometry method is more reliable at higher concentrations of the inhibitors. We showed that to determine precisely the inhibition efficacy the flow cytometry is better; hence, to get the correct E_max and EC₅₀ values, we cannot only rely on the MPR. Conclusion. Both techniques can potentially be used extensively in the pharmaceutical industry for high-throughput drug screening and in biology laboratories for academic research, monitoring the P-gp efflux in specific assays.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848087

DOI: 10.1155/2013/676845
PubMed: 24350282
PubMed Central: 3848087

Affiliations:

Le document en format XML

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<author><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Line, Tumor</term>
<term>Drug Resistance, Multiple (physiology)</term>
<term>Drug Resistance, Neoplasm (physiology)</term>
<term>Fluoresceins (metabolism)</term>
<term>Fluorescence</term>
<term>Humans</term>
<term>MCF-7 Cells</term>
<term>P-Glycoproteins (metabolism)</term>
<term>Reproducibility of Results</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fluoresceins</term>
<term>P-Glycoproteins</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Drug Resistance, Multiple</term>
<term>Drug Resistance, Neoplasm</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cell Line, Tumor</term>
<term>Fluorescence</term>
<term>Humans</term>
<term>MCF-7 Cells</term>
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<front><div type="abstract" xml:lang="en"><p><italic>Background</italic>
. P-gp expression has been linked to the efflux of chemotherapeutic drugs in human cancers leading to multidrug resistance. Fluorescence techniques have been widely applied to measure the P-gp activity. In this paper, there is a comparison between the advantages of two fluorescence approaches of commonly available and affordable instruments: the microplate reader (MPR) and the flow cytometer to detect the P-gp efflux activity using calcein-AM. <italic>Results</italic>
. The selectivity, sensibility, and reproducibility of the two methods have been defined. Our results showed that the MPR is more powerful for the detection of small inhibition, whereas the flow cytometry method is more reliable at higher concentrations of the inhibitors. We showed that to determine precisely the inhibition efficacy the flow cytometry is better; hence, to get the correct <italic>E</italic>
<sub>max</sub>
 and EC<sub>50</sub>
 values, we cannot only rely on the MPR. <italic>Conclusion</italic>
. Both techniques can potentially be used extensively in the pharmaceutical industry for high-throughput drug screening and in biology laboratories for academic research, monitoring the P-gp efflux in specific assays.</p>
</div>
</front>
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<author><name sortKey="Zittoun, R" uniqKey="Zittoun R">R Zittoun</name>
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<author><name sortKey="Marie, Jp" uniqKey="Marie J">JP Marie</name>
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</analytic>
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<author><name sortKey="Hollo, Z" uniqKey="Hollo Z">Z Hollo</name>
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<author><name sortKey="Germann, Ua" uniqKey="Germann U">UA Germann</name>
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<author><name sortKey="Pastan, I" uniqKey="Pastan I">I Pastan</name>
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<author><name sortKey="Gottesman, Mm" uniqKey="Gottesman M">MM Gottesman</name>
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<author><name sortKey="Sarkadi, B" uniqKey="Sarkadi B">B Sarkadi</name>
</author>
</analytic>
</biblStruct>
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<author><name sortKey="Legros, H" uniqKey="Legros H">H Legros</name>
</author>
<author><name sortKey="Poret, A" uniqKey="Poret A">A Poret</name>
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<author><name sortKey="Leboulenger, F" uniqKey="Leboulenger F">F Leboulenger</name>
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<author><name sortKey="Le Foll, F" uniqKey="Le Foll F">F Le Foll</name>
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<author><name sortKey="Magal, P" uniqKey="Magal P">P Magal</name>
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<author><name sortKey="Boulange Lecomte, C" uniqKey="Boulange Lecomte C">C Boulangé-Lecomte</name>
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<author><name sortKey="Webb, G" uniqKey="Webb G">G Webb</name>
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<author><name sortKey="Bland, Jm" uniqKey="Bland J">JM Bland</name>
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</author>
<author><name sortKey="Marie, J P" uniqKey="Marie J">J-P Marie</name>
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<li>États-Unis</li>
</country>
<region><li>Haute-Normandie</li>
<li>Région Normandie</li>
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<tree><country name="France"><region name="Région Normandie"><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name>
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<name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name>
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<country name="États-Unis"><region name="État de New York"><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name>
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<country name="Qatar"><noRegion><name sortKey="Pasquier, Jennifer" sort="Pasquier, Jennifer" uniqKey="Pasquier J" first="Jennifer" last="Pasquier">Jennifer Pasquier</name>
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<name sortKey="Abu Kaoud, Nadine" sort="Abu Kaoud, Nadine" uniqKey="Abu Kaoud N" first="Nadine" last="Abu-Kaoud">Nadine Abu-Kaoud</name>
<name sortKey="Guerrouahen, Bella S" sort="Guerrouahen, Bella S" uniqKey="Guerrouahen B" first="Bella S." last="Guerrouahen">Bella S. Guerrouahen</name>
<name sortKey="Rafii, Arash" sort="Rafii, Arash" uniqKey="Rafii A" first="Arash" last="Rafii">Arash Rafii</name>
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</tree>
</affiliations>
</record>

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{{Explor lien
   |wiki=    Wicri/France
   |area=    LeHavreV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:3848087
   |texte=   P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods
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P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods

P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods

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